ADDITION OF MANGOSTEEN RIND (Garcinia mangostana L.) SIMPLICIA IN FEED ON DIFFERENTIATION OF LEUKOCYTES OF AFRICAN CATFISH (Clarias gariepinus) INFECTED BY Aeromonas hydrophila

Abstract

This research was conducted on 12nd May until 23rd July 2015. The aims of this
research was to determine the best dose of mangosteen rind (Garcinia mangostana
L.) simplicia addition in feed to increase non-specific immune responses in African
catfish (Clarias gariepinus). This research used Completely Randomized Design
(CRD) with one factor, five treatments and three replications. The treatments were
addition of mangosteen rind simplicia in feed with different concentration i.e : Kn
(without addition of mangosteen rind simplicia and without infected with A.
hydrophila), Kp (without addition of mangosteen rind simplicia and infected with A.
hydrophila), P1 (addition of mangosteen rind simplicia 2 g/kg of feed and infected
with A. hydrophila), P2 (addition addition of mangosteen rind simplicia 4 g/kg of feed
and infected with A. hydrophila) and P3 (addition of mangosteen rind simplicia 6 g/kg
of feed and infected with A. hydrophila). The results showed that mangosteen rind
simplicia in feed of treatment P3 on the best dose (6 g/kg of feed) and it can be
increase of non-specific immune responses in African catfish.
Key words : Garcinia mangostana L., Clarias gariepinus, A. hydrophila,
Simplicia, Differentiation of Leukocytes