TOTAL FENOLIK,FLAVONOID SERTA AKTIVITAS ANTIOKSIDAN EKSTRAK n-HEKSANA, DIKLOROMETAN DAN METANOL Amaranthus spinosus L EM5-BAWANG PUTIH

Abstract

The objective of this study was to analyze the total phenolics, total flavonoids, and antioxidant activities of Amaranthus spinosus L leaves. During cultivation, these plants were treated with 2 treatments, biocontrol Effective Microorganism 5 (EM5) garlic and control (water). The A. spinosus L leaves were dried using freeze dryer and extracted by dissolving n-hexane, dicloromethane (DCM) and methanol. The results showed that A.spinosus L leaves treated with biocontrol had the highest total phenolic, total flavonoid and antioxidant activity content compare to the control. The total phenolic, total flavonoid, 2,2 diphenyl-1-picrylhydrazyl (DPPH) and Nitric Oxide (NO) radical scavenging were the highest on DCM extract with each value of 74,656 mg Gallic Acid Equivalent (GAE)/g of dry weight (DW), 22,337 mg Catechin Equivalent (CE)/g DW, 0,373 mg/mL and 0,025 mg/mL. n-Hexane extract had the highest antioxidant activity on Ferric Thiocyanate (FTC) test (27,615 %), meanwhile methanol extract indicated the highest antioxidant activity on FRAP test (1,022 mmol Fe+2/g DW). From this study, it can be concluded that A. spinosus L leaves with biocontrol Effective Microorganism 5 (EM5) garlic can increase the phenolic, flavonoids and antioxidant activitiy contents of A. spinosus L plant.