ISOLASI DAN KARAKTERISTIK BAKTERI LIMBAH LUMPUR MINYAK BUMI (OIL SLUDGE) DI AREA PT PERTAMINA KECAMATAN LIRIK SEBAGAI RANCANGAN LEMBAR KERJA PESERTA DIDIK BIOLOGI SMA

Abstract

Abstract: This research was conducted to determine the bacteria characteristics
of oil sludge waste in the area of PT Pertamina, Lirik district and its potential to
produce student worksheet designs with subject characteristics of bacteria in class X
SMA KD 3.5 and 4.5. This research was conducted from December 2018 to March
2019 at the Microbiology Laboratory, Faculty of Medicine, University of Riau. This
study used two stages the first stage of research using qualitative descriptive to
determine the characteristics of bacteria in oil sludge waste. The parameters of this
study are characteristics of bacteria isolated from samples of oil sludge waste in Lirik
District through macroscopic observations (colony shape, colony margin, colony color,
and colony elevation), and microscopic (gram stain and cell shape). Phase II research
is the design of student worksheet teaching subject that uses two stages, those are
analysis and design stages. The results showed that the bacteria found had the
characteristics of round, irregular, and filamentous. The elevations found are raised,
flat, and umbonate. The margin of the colonies found were flat and lobate. The colors of
the colonies found were white and yellow. The most common form of cells found was
rods, the most common gram found was gram negative. And the most common cell color
found is purple. The results of this study can be used as a learning resource in the form
of a student worksheet design for the subject characteristics of bacteria class of X in
High School.
Key Words : Isolation, Characteristics, Worksheets