KORELAS I NILAI ABSORBAN Fe 2 + TERHADAP US IA BERCAK DARAH YANG DIANALI S I S DENGAN MENGGUNAKAN S PEKTROFOTOMETRI UV- Vi s

Abstract

Bloodstains is one of the biological fluids are most commonly found on
the crime scene criminal cases. In the blood, there are 3 (three) components:
erythrocytes, leukocytes, and plasma, iron (Fe) within the hemoglobin in
erythrocytes can be utilized in the estimate of the age of bloodstain. At the
moment the blood into blood, iron contained in hemoglobin (Fe2+) will react
with the oxygen present in nature which will oxidize Fe2+ to Fe3+, The process
of changing Fe2+ to Fe3+ can be used to estimate the age of bloodstain at the
scene. One of them is by using UV-Vis spectrophotometry. This study aimed to
determine the correlation of absorbance (Å) Fe2+ to the age of bloodstain by
using UV-Vis spectrophotometry. This study is an experimental, Samples to be
used as research is a white cotton cloth soaked in human blood. The results
show that no correlation was found from absorbance (Å) Fe2+ with the age of
bloodstain were analized using UV-Vis spectrophotometry.
Keyword : Absorbance Fe2+, UV-Vis spectrophotometry, Bloodstain