THE EFFECT OF DIFFERENT TEMPERATURES ON THE ACTIVITY OF THE CRUDE EXTRACT OF THE COLLAGENASE ENZYME IN THE INTERNAL ORGANS OF SALINE TILAPIA (Oreochromis niloticus)

Abstract

ABSTRACT

Enzymes are biocatalysts that function to accelareta the rate of a chemical reaction without being involved in the reaction. Collagenase is a proteolytic enzyme capable of degrading polypeptide bonds. The activity of the enzyme hydrolyzing protein is influenced by several factors, namely temperature, pH, substrate, inhibitor, and activator. This study was aimed to determine the effect of temperature differences on the activity of the crude extract of the collagenase enzyme from the internal organs of saline tilapia (Oreochromis niloticus). This research method is a completely randomized design (CRD) non-factorial. The treatments used were different temperatures of 45ºC, 55ºC, and 65ºC. The parameter of the analysis is the activity of crude extract of collagenase enzyme from the internal organs of saline tilapia (Oreochromis niloticus). The results showed that the internal organs of saline tilapia (Oreochromis niloticus) had crude extract activity of the collagenase enzyme at 45ºC, 55ºC, and 65ºC 0.297 U/mL, and 0.272 U/mL, respectively. The highest crude extract activity of collagenase enzyme was 0.326 U/ml at 55ºC

 Keywords :  activity, enzyme, extract, collagenase, temperature